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101.
Both thick- and thin-walled sieve tubes in leaf-blade veins of Hordeum vulgare L. exhibit a distinct, electron-opaque inner wall layer after fixation in glutaraldehyde-osmium tetroxide and staining with uranyl acetate and lead citrate. This inner wall layer is thickest at the sieve plates and lateral sieve areas where it is permeated by a labyrinth of tubules formed by the plasmalemma. Along the lateral walls between sieve areas the inner wall layer apparently is penetrated by numerous microvilli-like evaginations of the plasmalemma, giving the cell wall-plasmalemma interface the appearance of a brush border. It is suggested that a similar brush-border-like structure may occur at the cell wall-plasmalemma interface of sieve elements in a wide variety of vascular plants.Abbreviation ER
endoplasmic reticulum 相似文献
102.
Valerie Vreeland Suzanne R. Morse Robert H. Robichaux Kathleen L. Miller Sui-Sheng T. Hua Watson M. Laetsch 《Planta》1989,177(4):435-446
Carbohydrate-hybridization probes (Vreeland and Laetsch, 1989, Planta (177, 423–434) were used to localize the homogalacturonan (pectate) component of pectins in the cell walls of leaves and soybean root nodules. Leaves of two species of the dicotyledon Dubautia were compared; these species contain much pectin but differ in their tissue water relations with respect to their cell-wall properties. Maturation of the primary cell walls in nodules was studied in the Bradyrhizobium japonicum-Glycine max symbiosis. Probe labelling was based on the divalent-cation-mediated association between pectate in tissue sections and fluorescein-conjugated pectate fragments. Pectate was also labelled by mixed-dimer formation with fluorescent polyguluronate derived from alginate. The specificity of the probe for unesterified polygalacturonate was indicated by increased cell-wall labelling after chemical or enzymatic deesterification of tissue sections, in contrast to elimination of labelling by chemical esterification. Postfixation of tissue sections improved retention of soluble pectate. Pectate differences were found in the leaves among cell types, in degree of esterification, and between plant species. The cell walls of soybean nodules were strongly labelled by the pectate probe in nodules one week and three weeks after infection. Pectate was more highly esterified in the central infected zone than in the surrouding cortex. Within the infected zone, walls of uninfected cells and infected cells were similarly labelled by the pectate probe. The results indicate that the pectate molecular probe provides detailed information on pectate distribution at the cellular level for investigations of cell-wall structure, development and physiology.Abbreviations EDTA
ethylenedinitrilotetraacetic acid (ethylenediaminetetraacetic acid)
- NMR
nuclear magnetic resonance spectroscopy
- TTB
1,3,5-triazido-2,4,6-trinitrobenene 相似文献
103.
Hiroshi Ihobe 《Primates; journal of primatology》1989,30(1):17-25
When the individual Japanese macaques of the Koshima troop feed on natural food, they usually feed alone. In situations where
animals usually feed without other animals, there is a possibility that subordinate animals may avoid feeding sites at which
dominant animals are feeding. This paper examines whether social relationships such as kinship or dominance exert any influence
on an animal's choice of feeding sites, by analyzing episodes in which an animal approached and climbed into a tree where
other animals were. As a result, it was found that social relationships did not influence whether an animal climbed into a
tree where other animals were feeding, and that no particular age-sex pair co-fed. Agonistic interactions frequently occurred
when the inter-individual distance was less than 1 m. From these findings, the feeding sites were divided into two spaces:
(1) a tolerance feeding space, and (2) an intolerance feeding space. It is presumed that animals can feed without entering
others' intolerance feeding spaces when food is abundant, as it was in the present study period. Thus social relationships
do not influence an animal's choice of feeding sites in such a situation. 相似文献
104.
Jane J. Turner James G. Herndon Maria-Carmen Ruiz de Elvira Delwood C. Collins 《Primates; journal of primatology》1989,30(4):523-529
Endogenous testosterone levels were measured in association with sexual, aggressive, and social/affiliative behaviors in 11
outdoor-housed female rhesus monkeys over a ten-month period. Several behaviors (sex directed toward the male, sex received
from the male, aggression directed toward the male, submission directed toward the male, submission directed toward the female,
and groom another female) were significantly (p<0.05) positively correlated with testosterone in from one to five females. No trends were strong enough across all females
to suggest that any of these correlations have species-wide significance. Factor analysis revealed clearcut clusters of behaviors,
but elevations in testosterone were not strongly associated with any of these clusters. It is concluded that endogenous testosterone
levels have little measurable effect on overt behavior in female rhesus monkeys. 相似文献
105.
Summary Cell wall regeneration around protoplasts from Black Mexican Sweet corn suspension cells has been observed using scanning electron microscopy. A coherent array of cellulose microfibrils can be seen around protoplasts two hours after they have been isolated. This array does not form in the presence of 15 mg/l Congo Red. The frequency and electrical resistance of seals made between patch clamp pipettes and the plasmalemma around corn protoplasts is not significantly affected by the presence or absence of these fibrils (p0=0.75); it remains relatively low. Some single channel records from BMS corn protoplasts are shown. 相似文献
106.
Summary Uniseriate filaments of the freshwater red algaCompsopogon coeruleus were examined by transmission electron microscopy for details of vegetative organization and cell division with the goal of providing useful taxonomic characters. Each cell's single, complex chloroplast contains a peripheral encircling thylakoid, and unlike the vast majority of red algae, the cis-regions of dictyosomes are not consistently juxtaposed with mitochondria. These subcellular features, which are present in all examined genera in theCompsopogonales, Erythropeltidales, andRhodochaetales, along with certain unique reproductive characteristics, unify these three orders. During mitosis in uncorticated axial cells, a small, ring-shaped nucleus associated organelle (NAO) is located at each division pole, an intranuclear spindle comes to a moderately acute focus at the flattened, fenestrated metaphase-anaphase division poles and perinuclear ER partially encloses dividing nuclei, including a well-developed interzonal midpiece. The cleavage furrow penetrates the large, central vacuolar region to separate daughter nuclei. These cell division features most closely resemble the pattern described for the orderCeramiales. Our observations of vegetative and dividing cells ofC. coeruleus supplement the growing volume of evidence in favour of uniting all red algae into a single class without subclass designations.Abbreviations ER
endoplasmic reticulum
- IZM
interzonal midpiece
- MT
microtubule
- MTOC
microtubule organizing center
- NAO
nucleus associated organelle
- NE
nuclear envelope
- PER
perinuclear endoplasmic reticulum 相似文献
107.
108.
H. V. Cornell 《Evolutionary ecology》1989,3(1):64-76
Summary Endophagous folivores, which are concealed inside leaf tissue for much of their life cycle, or which live externally but feed internally, should be more successful on heavily defended plants than ectophagous species. This is because endophages are more facile at feeding selectively and can manipulate tissue development to avoid physical and chemical defenses and to enhance nutrition. As a result, endophage-ectophage ratios should increase on more heavily defended hosts. This pattern will likely be strengthened by negative asymmetrical interactions with ectophages and pathogens, which may displace endophages from lightly defended hosts. The hypothesis predicts that endophages should be particularly abundant in resource-poor habitats which seem to support a preponderance of heavily defended hosts. Although data do not yet exist for a rigorous test, several observed cases where endophage distributions seem biased toward heavily defended hosts are at least consistent with the hypothesis. Plant defense levels may have little influence on the total number of herbivores associated with a host, but I suggest that guild structure can be profoundly altered. 相似文献
109.
Dr. S. Forsgren 《Cell and tissue research》1989,256(1):125-135
Summary Recent studies have accomplished the establishment of a collagenous fiber-fringe matrix upon dental root surfaces in vitro. The present study was undertaken to follow the development of such a matrix in vitro and to test the possible effects of root surface treatments upon this matrix. Periodontal ligament cells, 0.1 to 0.2-mm thick dental root discs, and alveolar bone cells were derived after extraction from four partially erupted third molars and the accompanying interradicular bony septa of 1 male patient. Autologous serum was obtained by venipuncture. Cultures were initiated by delivering a 1-ml suspension of 50000 tritiated thymidine-labeled periodontal ligament cells and 50000 alveolar bone cells onto each of 42 culture sets. The following day, demineralized or non-demineralized root discs treated with autologous serum, fibronectin or complete medium were placed in pairs, separated by a 0.1–1.0 mm gap, upon the initial cell layer. Representative cultures were terminated after 2, 3, 4, 5 and 6 weeks, and processed for light- and electron microscopy, morphometric analysis and autoradiography. An outstanding feature of the early cultures (2, 3 and 4 weeks) was a patchwise, random distribution of matrix making a precise developmental study impossible, although collagen fibrils were produced within the first 2 weeks. Some 3-week cultures already demonstrated a mature fiber-fringe characterized electron-microscopically as oriented, densely packed collagen fibrils closely abutting the cementum-lined root discs. The treatments (including autologous serum) used in this study had no appreciable morphologic or morphometric effect upon the fiber-fringe formed. Because none of the cultures in the present or past studies have demonstrated a true cementoid matrix, this model may not be suitable for the in-vitro study of cementum formation. 相似文献
110.
Carey P. Yeager 《International journal of primatology》1989,10(6):497-530
Proboscis monkey (Nasalis larvatus) feeding behavior and ecology were studied at the Natai Lengkuas Station, Tanjung Puting National Park, Kalimantan Tengah,
Indonesia. Data on feeding behavior were collected using scan sampling during group follows. Three vegetational plots containing
1,732 trees were established and monitored monthly for changes in fruit, flower, and young leaf production. Basal area and
canopy cover were calculated and used in estimating food abundance. Proboscis monkeys were found to be folivore/frugivores,
specializing in seed consumption. At least 55 different plant species were used as food sources, with a marked preference
for Eugenia sp. 3/4,Ganua motleyana and Lophopetalum javanicum. These tree species were among the most frequent and most dominant. However, proboscis monkeys were selective feeders; use
of tree species as food sources was not based simply on relative density. During times of low food abundance and/or availability
proboscis monkeys switched dietary strategies and increased dietary diversity. The average total home range was estimated
to be 130.3 ha, with an average group density of 5.2 groups per km2. The average biomass per km2 was estimated to be 499.5 kg. Given their high biomass and predilection for consuming seeds of dominant species, proboscis
monkeys may help to maintain and increase vegetational diversity. 相似文献